Repeat-Specific Functions for the C-Terminal Domain of RNA Polymerase II in Budding Yeast

Functions of Mediator and the RNA Polymerase II C-terminal Domain in TranscriptiFunctions of Mediator and the RNA Polymerase II C-terminal Domain in Transcription Initiation

Disengaging polymerase: Terminating RNA polymerase II transcription in budding yeast☆

Termination of transcription by RNA polymerase II requires two distinct processes: The formation of a defined 3' end of the transcribed RNA, as well as the disengagement of RNA polymerase from its DNA template. Both processes are intimately connected and equally pivotal in the process of functional messenger RNA production. However, research in recent years has elaborated how both processes can...

Phosphorylation induces sequence-specific conformational switches in the RNA polymerase II C-terminal domain

The carboxy-terminal domain (CTD) of the RNA polymerase II (Pol II) large subunit cycles through phosphorylation states that correlate with progression through the transcription cycle and regulate nascent mRNA processing. Structural analyses of yeast and mammalian CTD are hampered by their repetitive sequences. Here we identify a region of the Drosophila melanogaster CTD that is essential for P...

Evolutionary diversity and taxon-specific modifications of the RNA polymerase II C-terminal domain.

In model eukaryotes, the C-terminal domain (CTD) of the largest subunit of DNA-dependent RNA polymerase II (RNAP II) is composed of tandemly repeated heptads with the consensus sequence YSPTSPS. The core motif and tandem structure generally are conserved across model taxa, including animals, yeasts and higher plants. Broader investigations revealed that CTDs of many organisms deviate substantia...

Interplay of positive and negative effectors in function of the C-terminal repeat domain of RNA polymerase II.

RNA polymerase II lacking a C-terminal domain (CTD) was active in transcription with purified proteins from yeast but failed to support transcription in a yeast extract. CTD dependence could be reconstituted in the purified system by addition of two fractions from the extract. An inhibitory fraction abolished transcription by both wild-type and CTD-less RNA polymerases; a stimulatory fraction r...